ANÁLISE MICROBIOLÓGICA DOS AGROBIOLÓGICOS UTILIZADOS EM PLANTAÇÕES DE CAFÉ

Alexsander Eduardo dos Santos Pedro; Gabriel Mendes de Freitas Lisboa; Paula Alves Pereira; Polyana Placedino Andrade; Hadassa Cristhina de Azevedo Soares dos Santos

doi:10.36674/mythos.v22i1.843

Authors

Alexsander Eduardo dos Santos Pedro Centro Universitário do Sul de Minas - UNISMG
Gabriel Mendes de Freitas Lisboa Centro Universitário do Sul de Minas - UNISMG
Paula Alves Pereira Centro Universitário do Sul de Minas - UNISMG
Polyana Placedino Andrade Centro Universitário do Sul de Minas - UNISMG
Hadassa Cristhina de Azevedo Soares dos Santos Centro Universitário do Sul de Minas - UNISMG

DOI:

https://doi.org/10.36674/mythos.v22i1.843

Keywords:

microorganisms, Coffee arabica, soil, microbiological analysis

Abstract

The soil plays a vital role in agriculture, being frequently enriched with a variety of products not naturally found in the environment to promote both environmental protection and vegetal growth control. Throughout agricultural development, there has been a gradual transition from the use of pesticides to the utilization of bacteria and other microorganisms known as agrobiologicals. These microorganisms act in symbiosis with plants, promoting their growth and protecting the soil. Additionally, they perform various beneficial functions, including nutrient cycling, nitrogen fixation, degradation of pollutants, and solubilization of minerals such as phosphorus, potassium, and sodium. Moreover, they exert biological control over diseases and pests, contributing to the health of the agricultural ecosystem. The aim of this study was to understand the utilization of agrobiologicals and evaluate the microorganisms present in these products, through microbiological analyses, in coffee plantations in the Southern region of Minas Gerais. The results revealed the predominant presence of genera such as Bacillus, Chromobacterium, Beauveria, Isaria, Hirsutella, Metarhizium, and Trichoderma in the agrobiologicals used for coffee cultivation in the region. Considering the potential negative impacts of pesticides on environmental contamination and chemical pollution, the substitution with agrobiologicals emerges as a promising alternative, capable of bringing significant ecological advantages and potentially benefiting public health.

Author Biographies

Alexsander Eduardo dos Santos Pedro, Centro Universitário do Sul de Minas - UNISMG

Bachelor's degree from Centro Universitário do Sul de Minas - UNISMG.

Gabriel Mendes de Freitas Lisboa, Centro Universitário do Sul de Minas - UNISMG

Specialist at Centro Universitário do Sul de Minas - UNISMG.

Paula Alves Pereira, Centro Universitário do Sul de Minas - UNISMG

Bachelor's degree from Centro Universitário do Sul de Minas - UNISMG.

Polyana Placedino Andrade, Centro Universitário do Sul de Minas - UNISMG

Agronomist graduated from the Federal University of Lavras (2008). Master's degree in Agronomy/Phytotechnology working with liming in sugarcane (2010) and PhD in Agronomy/Phytotechnology at the Federal University of Lavras (2013), working with Molecular Markers in sugarcane cultivation. Post-doctorate in Agronomy/Phytotechnology at the Federal University of Lavras, working on electrophoretic analysis of isoenzymes and quantification of enzyme activity in Elisa spectrometers in coffee seeds and beans (2014). Degree in Pedagogical Training for Non-Licensed Graduates at the Federal Institute of Education, Science and Technology of Southern Minas Gerais, IFSULDEMINAS, Pouso Alegre (2020). She worked as a Substitute Professor in Agronomy/Phytotechnics at the Federal Institute of Education, Science and Technology of Southern Minas Gerais - Câmpus Machado (July 2014 to December 2015) in the courses of Technologist in Agriculture, Agronomic Engineering and Higher Technology Course in Coffee Growing and Substitute Professor at the Federal Institute of Education, Science and Technology of Southern Minas Gerais - Câmpus Machado (July 2014 to December 2015) in the courses of Technologist in Agriculture, Agronomic Engineering and Higher Technology Course in Coffee Growing. Substitute Professor at the Federal Institute of Education, Science and Technology of Southern Minas Gerais - Muzambinho Campus (January to July 2016) in the courses of Technologist in Agriculture, Agronomic Engineering, Biology, Higher Technology Course in Coffee Growing and in the Lato Sensu Postgraduate Course in Coffee Growing. She also worked as a teacher at CESEP (2015 to 2016) in Machado on the Environmental Engineering and Agronomy courses. She worked as a distance learning teacher on the Technical Course in Coffee Growing (2018), as a distance learning tutor on the Technical Course in Coffee Growing (2017 to date) and on the Lato Sensu Postgraduate Course in Cachaça de Alambique Technology - TCA (2022). Currently Professor at Unis/MG (2017 to date) on the Agronomic Engineering course, Coordinator of the Agronomic Engineering course at Unis/MG, Coeditor of the Revista Agroveterinária do Sul de Minas in Varginha-MG and Member of the Research Department at Unis/MG. As of January 2025, she has been appointed by Unis as a full member of the Agronomy Sector Chamber of CREA/MG.

Hadassa Cristhina de Azevedo Soares dos Santos, Centro Universitário do Sul de Minas - UNISMG

Graduated in Biomedicine with a major in Clinical Analysis from Centro Universitário do Sul de Minas (2008). Has experience in Clinical Analysis (2008-2009). Further training in Microbiology at the University Hospital-USP (2009). Master's degree (2012) and PhD (2016) in Sciences (Faculty of Pharmacy/Department of Clinical and Toxicological Analysis) from the University of São Paulo. Post-doctoral student in Microbiology Teaching at the Institute of Biomedical Sciences of the University of São Paulo - ICB/USP. Research area with emphasis on microbiology, management, scientific dissemination and scientific areas of Medicine and Health Data Science. She is currently a researcher at the Centro Universitário do Sul de Minas (UNIS - Brazil) and project manager at the Faculty of Medicine of the University of Porto (FMUP - Portugal).

References

Angelo, P. D. S., Sena, M., Moraes, L., De Souza, M. G., Souza, A., & Lozano, J. (2007). Interação antagônica entre Chromobacterium violaceum e Colletotrichum guaranicola, agente causador da antracnose do guaraná. Disponível em: https://bit.ly/4hkRma9

Brito, P. F. D., Gomide, M., & Câmara, V. D. M. (2009). Agrotóxicos e saúde: realidade e desafios para a mudança de práticas na agricultura. Physis: Revista Saúde Coletiva, 19, 207-225. https://doi.org/10.1590/S0103-73312009000100011

Cavalcante, J. K. G., Conte, H., Daquila, B. V., & Caleffe, R. R. T. (2022). Potencial biotecnológico de Chromobacterium subtsugae: uma breve revisão focada no manejo biológico de artrópodes-praga. RevistaIbero-Americana de Ciências Ambientais, 13(3), 200-211. https://doi.org/10.6008/CBPC2179-6858.2022.003.0016

Conceschi, M. R. (2013). Potencial dos fungos entomopatogênicos Isaria fumosorosea e Beauveria bassianapara o controle de pragas da citricultura (Tese de doutorado, Universidade de São Paulo - USP).

Soares, A. C. F., Evangelista-Barreto, N. S., & Marbach, P. A. S. (Orgs.). (2020). Tópicos em microbiologia agrícola. EDUFRB. Disponível em: https://www1.ufrb.edu.br/editora/component/phocadownload/category/2-e- books?download=211:topicos-em-microbiologia-agricola

Ferraz, Helvio G. M. 2019. Método de diluição em placa para contagem de células bacterianas multiplicadas usando tecnologia multibacter®.

Ferreira, Ana Lúcia. 2016. Fungos e bactérias fazem as plantas crescerem mais. Embrapa. Rio de Janeiro. https://bit.ly/41FBp8I

Garcia, S. D., & de Lara, T. I. D. C. (2020). O impacto do uso de agrotóxicos na saúde pública: revisão de literatura. Saúde e Desenvolvimento Humano, 8(1), 85-96. https://doi.org/10.18316/sdh.v8i1.6087

Mariano, R. D. L. R., da Silveira, E. B., de Assis, S. M. P., Gomes, A. M. A., Nascimento, A. R. P., & Donato, V. M. T. S. (2004). Importância das bactérias que promovem o crescimento e o biocontrole de doenças de plantas para uma agricultura sustentável. Anais da Academia Pernambucana de CiênciasAgrárias, 1, 89-111. Disponível em: https://ainfo.cnptia.embrapa.br/digital/bitstream/item/34111/1/AAPCA-V1-Revisao-04.pdf

Meyer, M. C., Mazaro, S. M., & da SILVA, J. C. (2019). Trichoderma: uso na agricultura. https://www.embrapa.br/busca-de-noticias/-/noticia/53541439/fungo-trichoderma-e-aliado-no-controle-biologico-de-doencas-em-culturas-agricolas

Monnerat, R., Montalvão, S. C. L., Martins, E. S., Queiroz, P., da Silva, E. Y. Y., Garcia, A. R. M., & Gomes, A. (2020). Manual para produção e controle de qualidade de produtos biológicos à base de bactérias do gênero Bacillus para uso na agricultura. https://ainfo.cnptia.embrapa.br/digital/bitstream/item/213246/1/documentos-36916.pdf

Moreira, F. M. S., & Siqueira, J. O. (2006). Microbiologia e bioquímica do solo (2ª ed.). Editora UFLA. https://www.esalq.usp.br/departamentos/lso/arquivos_aula/LSO_400%20Livro%20-%20Microbiologia%20e%20bioquimica%20do%20solo.pdf

Neves, P. M., & Hirose, E. (2005). Seleção de isolados de Beauveria bassiana para o controle biológico da broca do café, Hypothenemus hampei (Ferrari)(Coleoptera: Scolytidae). Entomologia Neotropical, 34 (1), 77-82. https://doi.org/10.1590/S1519-566X2005000100011

Oliveira, P. A., Giordian, I., & Baretta, C. R. D. M. (2019). Benefícios da microbiota do solo na agricultura. SB Rural, 11(227), 1–1. https://www.udesc.br/arquivos/ceo/id_cpmenu/1043/rural_227_15526669528945_1043.pdf

Ribeiro, R., Sei, F. B., & Leite, M. S. (2011). Bacillus subtilis: agente de controle biológico e promotor de crescimento vegetal. Jornal do Dia de Campo. http://www.diadecampo.com.br/zpublisher/materias/Materia.asp?id=24104&secao=Colu n

Vasconcelos, J. F., Silva, S. S., Santana, S. F., & Teodoro, A. V. (2015). Eficiência relativa de agrotóxicos, óleo de algodão e fungo Hirsutella thompsonii no controle do ácaro da necrose Aceria guerreronis. Em Anais do V Seminário de Iniciação Científica e Pós-Graduação da Embrapa Tabuleiros Costeiros (pp. 248–253). Embrapa. https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1028234/1/Eficienciarelativa.pdf

Veloso, C. (2023 Entenda a importância da microbiota do solo e da inoculação de microrganismos. Bloco Verde. https://blog.verde.ag/pt/nutricao-de-plantas/entenda-a-importancia-da-microbiota-do-solo-e-da-inoculacao-de-microrganismos/

Veloso, C. (2023). Preservar a microbiota do solo pode aumentar a produtividade e a rentabilidade da sua cultura? Bloco Verde. https://blog.verde.ag/pt/nutricao-de-plantas/preservar-a-microbiota-do-solo-pode-aumentar-produtividade-e-rentabilidade-da-sua-lavoura/